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electrocompetent_cells [2022/07/14 21:34]
moth
electrocompetent_cells [2022/07/14 21:34] (current)
moth
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 For any strains available, we offer electrocompetent cells.  For any strains available, we offer electrocompetent cells. 
  
-===== Electrocompetent cell prep can be tedious & unrewarding. We have a few protocols available. +===== Electrocompetent cell prep can be tedious & unrewarding. We have a few protocols available.=====
- =====+
  
 In the purest form, you only need to collect cells at a high density without contaminants and in 10% glycerol. We recommend starting from fresh 2 day old cultures, or collecting from a 2 day old plate.  In the purest form, you only need to collect cells at a high density without contaminants and in 10% glycerol. We recommend starting from fresh 2 day old cultures, or collecting from a 2 day old plate. 
  
 Washing many times with sterile water is often easier than using glycerol. The final wash and remainder of the prep should be 10% glycerol. While lower temperatures during the process is common in the literature, we have found room temperature is sufficient.  Washing many times with sterile water is often easier than using glycerol. The final wash and remainder of the prep should be 10% glycerol. While lower temperatures during the process is common in the literature, we have found room temperature is sufficient. 
/home/agrokwev/public_html/data/pages/electrocompetent_cells.txt · Last modified: 2022/07/14 21:34 by moth