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--- electrocompetent_cells [2022/02/12 18:23]
moth created
+++ electrocompetent_cells [2022/07/14 21:34]
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 For any strains available, we offer electrocompetent cells.
+===== Electrocompetent cell prep can be tedious & unrewarding. We have a few protocols available.
+ =====
+In the purest form, you only need to collect cells at a high density without contaminants and in 10% glycerol. We recommend starting from fresh 2 day old cultures, or collecting from a 2 day old plate.
+Washing many times with sterile water is often easier than using glycerol. The final wash and remainder of the prep should be 10% glycerol. While lower temperatures during the process is common in the literature, we have found room temperature is sufficient.

Agrobacterium.science