Agrobacterium.science

auxotrophs - [Auxotrophic strains offer two main advantages]

moth (moth@undisclosed.example.com) — 2022-07-15T02:03:32+00:00

Auxotrophy Auxotrophic strains offer two main advantages * Easier to remove from tissue culture * Higher transformation efficiencies When it comes to auxotrophy or strains that require medium supplementation, many examples exist. But certain mutations can be more favorable in one plant species versus another.

custom - [Custom strains]

moth (moth@undisclosed.example.com) — 2022-07-15T01:58:35+00:00

Custom strains We offer three broad services for creating custom Agrobacterium strains. These requests are typically for auxotrophy, removal of a host-range limiting factor, or editing genes controlling antibiotic resistance. Base editing Base editing is ideal for testing the role of different amino acids within proteins of Agrobacterium or disrupting expression of specific genes.

strain_testing

moth (moth@undisclosed.example.com) — 2022-07-15T01:57:27+00:00

Strain testing We realize that testing different Agrobacterium strains requires a large body of work. To help with this, we offer the follow services: Transformation Efficiency * Tobacco (N. benthamiana) * Switchgrass (P. virgatum, Alamo) *

custom_cloning - created

moth (moth@undisclosed.example.com) — 2022-07-15T01:51:57+00:00

Custom Cloning Cloning can get difficult, especially when companies can't synthesize a certain sequence or the plasmids get too large. We offer: * Custom plasmids up to 70kb * Plasmid design & advice (antibiotics, fusions, delivery, origins) *

ternary_vectors - created

moth (moth@undisclosed.example.com) — 2022-07-15T01:48:51+00:00

Ternary/Quaternary Vectors Ternary Vectors Following the work of Hamilton, CAMBIA, and Corteva, we have developed our own ternary vectors for improving transformations. Variations exist, and we like to cater our ternaries to the bacterial strain and plant species of interest.

binary_vectors - created

moth (moth@undisclosed.example.com) — 2022-07-15T01:45:20+00:00

Binary Vectors We have many common binary vectors, and can design and create a vector if none fit your criteria. We love the CAMBIA vectors. We also have many from the various 'gate' systems. If desired, we can create a vector. We have experience cloning vectors up to 70kb in size. We also offer visual markers so you can be sure your plasmid is in your

agrobacterium_libraries

moth (moth@undisclosed.example.com) — 2022-07-15T01:42:09+00:00

Libraries available We offer libraries of Agrobacteriumto help scientists find the right strain for their project. We often breed or select specific plant species for their tissue culture or transformation properties, but very few people consider the diversity within the Rhizobium.

electrocompetent_cells

moth (moth@undisclosed.example.com) — 2022-07-15T01:34:59+00:00

Electrocompetent cells For any strains available, we offer electrocompetent cells. Electrocompetent cell prep can be tedious & unrewarding. We have a few protocols available. In the purest form, you only need to collect cells at a high density without contaminants and in 10% glycerol. We recommend starting from fresh 2 day old cultures, or collecting from a 2 day old plate.

sidebar

moth (moth@undisclosed.example.com) — 2022-07-15T01:31:54+00:00

Agrobacterium.science * About us * FAQ Community Outreach * Education * Gall Identification Bacterial Services & Requests * Wild type & armed * Disarmed * Custom * Electrocompetent cells * Agrobacterium libraries Plasmids * Binary vectors * Ternary vectors * Custom cloning Plant Services & Requests * Strain Testing Strains & Collections * Strains * Public Collections Engineered Strains * Disarmed * Auxotrophs * recA

disarmed

moth (moth@undisclosed.example.com) — 2022-07-15T01:31:11+00:00

Disarmed strains The follow disarmed strains are available. These strains possess the ability to transfer T-DNA located on a binary plasmid and lack the ability to transfer their native T-DNA. These are disarmed, or for use in delivering your DNA of interest via binary or ternary systems.

gall_identification

moth (moth@undisclosed.example.com) — 2022-07-15T01:21:14+00:00

Galls can be formed for a myriad of reasons. Midges, Wasps, and other insects often form galls as part of their life cycle. A useful website for this is - If you have a gall you would like us to identify, email us - moth@agrobio.works. If we believe it to be from an

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moth (moth@undisclosed.example.com) — 2022-02-12T20:04:56+00:00

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moth (moth@undisclosed.example.com) — 2022-01-03T20:09:05+00:00

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moth (moth@undisclosed.example.com) — 2022-01-01T23:26:41+00:00

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moth (moth@undisclosed.example.com) — 2022-01-01T23:24:05+00:00

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moth (moth@undisclosed.example.com) — 2022-01-01T23:22:38+00:00