http://agrobacterium.science/ 2026-04-06T01:30:35+00:00 http://agrobacterium.science/ http://agrobacterium.science/lib/tpl/dokuwiki/images/favicon.ico text/html 2022-07-15T01:16:16+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=about_us&rev=1657847776&do=diff About us This site is a work in progress. While we have many of these strains, services, and knowledge available, we are currently hoping to secure funding to devote ourselves fully. Agrobacterium Science was founded in South Carolina & Georgia in 2022. We aim to preserve text/html 2022-07-15T01:42:09+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=agrobacterium_libraries&rev=1657849329&do=diff Libraries available We offer libraries of Agrobacteriumto help scientists find the right strain for their project. We often breed or select specific plant species for their tissue culture or transformation properties, but very few people consider the diversity within the Rhizobium. text/html 2022-07-15T02:03:32+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=auxotrophs&rev=1657850612&do=diff Auxotrophy Auxotrophic strains offer two main advantages * Easier to remove from tissue culture * Higher transformation efficiencies When it comes to auxotrophy or strains that require medium supplementation, many examples exist. But certain mutations can be more favorable in one plant species versus another. text/html 2022-07-15T01:45:20+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=binary_vectors&rev=1657849520&do=diff Binary Vectors We have many common binary vectors, and can design and create a vector if none fit your criteria. We love the CAMBIA vectors. We also have many from the various 'gate' systems. If desired, we can create a vector. We have experience cloning vectors up to 70kb in size. We also offer visual markers so you can be sure your plasmid is in your text/html 2022-07-15T01:58:35+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=custom&rev=1657850315&do=diff Custom strains We offer three broad services for creating custom Agrobacterium strains. These requests are typically for auxotrophy, removal of a host-range limiting factor, or editing genes controlling antibiotic resistance. Base editing Base editing is ideal for testing the role of different amino acids within proteins of Agrobacterium or disrupting expression of specific genes. text/html 2022-07-15T01:51:57+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=custom_cloning&rev=1657849917&do=diff Custom Cloning Cloning can get difficult, especially when companies can't synthesize a certain sequence or the plasmids get too large. We offer: * Custom plasmids up to 70kb * Plasmid design & advice (antibiotics, fusions, delivery, origins) * text/html 2022-02-10T23:37:47+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=custom_strains&rev=1644536267&do=diff Custom strains We offer two services for creating custom Agrobacterium strains. Base editing Base editing is ideal for testing the role of different amino acids within proteins of Agrobacterium or disrupting expression of specific genes. Homologous recombination text/html 2022-02-12T23:21:45+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=delivery&rev=1644708105&do=diff Delivery can be done through T3SS or T4SS. T3SS T4SS text/html 2022-02-10T23:33:21+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=difficult_cloning&rev=1644536001&do=diff Difficult cloning text/html 2022-07-15T01:31:11+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=disarmed&rev=1657848671&do=diff Disarmed strains The follow disarmed strains are available. These strains possess the ability to transfer T-DNA located on a binary plasmid and lack the ability to transfer their native T-DNA. These are disarmed, or for use in delivering your DNA of interest via binary or ternary systems. text/html 2022-07-15T01:34:59+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=electrocompetent_cells&rev=1657848899&do=diff Electrocompetent cells For any strains available, we offer electrocompetent cells. Electrocompetent cell prep can be tedious & unrewarding. We have a few protocols available. In the purest form, you only need to collect cells at a high density without contaminants and in 10% glycerol. We recommend starting from fresh 2 day old cultures, or collecting from a 2 day old plate. text/html 2022-07-15T01:17:48+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=faq&rev=1657847868&do=diff Frequently Asked Questions 1. Do you ship Agrobacterium strains? 2. Are these strains characterized? 3. Is this Agrobacterium? 4. What plasmids work in Agrobacterium? 5. Will Agrobacterium work for my crop? 6. Why is Agrobacterium important for plant biotechnology? text/html 2022-07-15T01:21:14+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=gall_identification&rev=1657848074&do=diff Galls can be formed for a myriad of reasons. Midges, Wasps, and other insects often form galls as part of their life cycle. A useful website for this is - <https://www.gallformers.org/> If you have a gall you would like us to identify, email us - moth@agrobio.works. If we believe it to be from an text/html 2022-02-08T20:05:59+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=get_involved&rev=1644350759&do=diff Email timchappelluga@gmail.com! text/html 2022-02-11T03:01:33+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=gv3101&rev=1644548493&do=diff GV3101 GV is an abbreviation of Genetische Virologie, the old name of the Ghent University department where the laboratories of Jeff Schell & Marc Van Montagu were located. The number 3101 refers to the strain number when originally deposited in the collection and was made by Marcel Holster who cured the pTiC58 Ti plasmid from the Rifampicin resistant strain C58. C1 stands for cured 1, MP is an abbreviation for Max-Planck. text/html 2022-01-02T23:47:05+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=hi&rev=1641167225&do=diff text/html 2022-02-10T23:33:10+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=identification&rev=1644535990&do=diff Identification text/html 2022-02-11T16:57:43+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=lba4404&rev=1644598663&do=diff LBA4404 LBA means “Lugdunum Batavorum Agrobacterium.” Pedigree: Ach5 aka LBA4001 (pTiAch5) -> LBA4013 (pAL102) -> LBA4213 (pAL231) -> LBA4404 (pAL4404) text/html 2022-02-12T23:45:22+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=media&rev=1644709522&do=diff Media Many mediums are useful when working Agrobacterium 1A selective M9 Acetosyringone Benedict's reagent Carbenicillin G418 Gentamycin Kanamycin LB Meropenem MES Rifampicin Spectinomycin Streptomycin Timentin Tetracycline YM text/html 2022-02-21T21:07:38+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=public_collections&rev=1645477658&do=diff Public Collections Finding particular Agrobacterium strains is difficult or impossible if the particular strain is not one of the commonly used strains. These collections are the best to search for strains that were once isolated but more difficult to find. text/html 2022-07-15T01:31:54+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=sidebar&rev=1657848714&do=diff Agrobacterium.science * About us * FAQ Community Outreach * Education * Gall Identification Bacterial Services & Requests * Wild type & armed * Disarmed * Custom * Electrocompetent cells * Agrobacterium libraries Plasmids * Binary vectors * Ternary vectors * Custom cloning Plant Services & Requests * Strain Testing Strains & Collections * Strains * Public Collections Engineered Strains * Disarmed * Auxotrophs * recA text/html 2022-02-10T23:53:57+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=start&rev=1644537237&do=diff Agrobacterium Science Agrobacterium.science is the homepage for all information relating to Agrobacterium. We hope to build community knowledge, and offer services to scientists in academia and industry. ---------- Why Agrobacterium.science? text/html 2022-07-15T01:57:27+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=strain_testing&rev=1657850247&do=diff Strain testing We realize that testing different Agrobacterium strains requires a large body of work. To help with this, we offer the follow services: Transformation Efficiency * Tobacco (N. benthamiana) * Switchgrass (P. virgatum, Alamo) * text/html 2022-02-12T22:44:48+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=strains&rev=1644705888&do=diff Strains As of 2022, we know of ~200 strains of Agrobacterium. We hope to maintain information, references, and availability on this page. C58 EHA101 EHA105 AGL1 LBA4404 GV3101 K599 R1000 T37 H100 text/html 2022-07-15T01:48:51+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=ternary_vectors&rev=1657849731&do=diff Ternary/Quaternary Vectors Ternary Vectors Following the work of Hamilton, CAMBIA, and Corteva, we have developed our own ternary vectors for improving transformations. Variations exist, and we like to cater our ternaries to the bacterial strain and plant species of interest. text/html 2022-02-28T00:39:51+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=wild_type_armed&rev=1646008791&do=diff Wild type strains The follow wild type strains are available. These strains possess the ability to transfer T-DNA that results in tumor or gall formation. They contain their original chromosomal & Ti plasmid. These are not disarmed, or for use in delivering your DNA of interest. text/html 2022-02-12T23:06:20+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=wild_type_armed_strains&rev=1644707180&do=diff Wild type strains The follow wild type strains are available. These strains possess the ability to transfer T-DNA that results in tumor or gall formation. They contain their original chromosomal & Ti plasmid. These are not disarmed, or for use in delivering your DNA of interest. text/html 2022-02-12T22:48:10+00:00 Anonymous (anonymous@undisclosed.example.com) http://agrobacterium.science/doku.php?id=wild_type_strains&rev=1644706090&do=diff Wild type strains The follow wild type strains are available. These strains possess the ability to transfer T-DNA that results in tumor or gall formation. They contain their original chromosomal & Ti plasmid. These are not disarmed, or for use in delivering your DNA of interest.