User Tools
electrocompetent_cells
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision | |||
|
electrocompetent_cells [2022/07/15 01:34] moth |
electrocompetent_cells [2022/07/15 01:34] (current) moth |
||
|---|---|---|---|
| Line 3: | Line 3: | ||
| For any strains available, we offer electrocompetent cells. | For any strains available, we offer electrocompetent cells. | ||
| - | ===== Electrocompetent cell prep can be tedious & unrewarding. We have a few protocols available. | + | ===== Electrocompetent cell prep can be tedious & unrewarding. We have a few protocols available.===== |
| - | ===== | + | |
| In the purest form, you only need to collect cells at a high density without contaminants and in 10% glycerol. We recommend starting from fresh 2 day old cultures, or collecting from a 2 day old plate. | In the purest form, you only need to collect cells at a high density without contaminants and in 10% glycerol. We recommend starting from fresh 2 day old cultures, or collecting from a 2 day old plate. | ||
| Washing many times with sterile water is often easier than using glycerol. The final wash and remainder of the prep should be 10% glycerol. While lower temperatures during the process is common in the literature, we have found room temperature is sufficient. | Washing many times with sterile water is often easier than using glycerol. The final wash and remainder of the prep should be 10% glycerol. While lower temperatures during the process is common in the literature, we have found room temperature is sufficient. | ||
/home/agrokwev/public_html/data/pages/electrocompetent_cells.txt · Last modified: 2022/07/15 01:34 by moth
Page Tools
