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Electrocompetent cells

For any strains available, we offer electrocompetent cells.

Electrocompetent cell prep can be tedious & unrewarding. We have a few protocols available.

In the purest form, you only need to collect cells at a high density without contaminants and in 10% glycerol. We recommend starting from fresh 2 day old cultures, or collecting from a 2 day old plate.

Washing many times with sterile water is often easier than using glycerol. The final wash and remainder of the prep should be 10% glycerol. While lower temperatures during the process is common in the literature, we have found room temperature is sufficient.

/home/agrokwev/public_html/data/pages/electrocompetent_cells.txt · Last modified: 2022/07/15 01:34 by moth