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electrocompetent_cells
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electrocompetent_cells [2022/02/12 23:23] moth created |
electrocompetent_cells [2022/07/15 01:34] (current) moth |
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| For any strains available, we offer electrocompetent cells. | For any strains available, we offer electrocompetent cells. | ||
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| + | ===== Electrocompetent cell prep can be tedious & unrewarding. We have a few protocols available.===== | ||
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| + | In the purest form, you only need to collect cells at a high density without contaminants and in 10% glycerol. We recommend starting from fresh 2 day old cultures, or collecting from a 2 day old plate. | ||
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| + | Washing many times with sterile water is often easier than using glycerol. The final wash and remainder of the prep should be 10% glycerol. While lower temperatures during the process is common in the literature, we have found room temperature is sufficient. | ||
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